Lokasyon ng Filming
127A Smithfield Road, Frederiksted, Virgin Islands
Training for stool-based qPCR diagnostics for soil-transmitted helminths developed by London Centre for Neglected Tropical Disease Research
Manood ng Video
Paglalarawan
The TV series Training for stool-based qPCR diagnostics for soil-transmitted helminths developed by London Centre for Neglected Tropical Disease Research, created by an unlisted creator and produced by an unlisted studio, first premiered on September 6, 2021 in the United Kingdom. The series spans 1 season with 5 episodes, featuring the voices of an unlisted voice cast. It follows the story of LCNTDR has developed and filmed a virtual training course including all components of qPCR soil-transmitted helminths diagnostics. The training is presented in a series of videos covering stool-based DNA extractions, pre-PCR and qPCR preparation, primer and probe resuspension for qPCR assays, qPCR software set-up (with a demo on a StepOne Plus instrument), and demo of a Python script for combining qPCR data; all demonstrated with explanatory instructions and detailed voice over by Marina Papaiakovou. Marina is an expert in qPCR diagnostics and member of WHO’s Diagnostics Technical Advisory Group (DTAG) for Manufacturing and Regulatory Pathways for neglected tropical diseases (NTDs). We intend for this resource to be shared freely and widely and hope teams across the globe will find it useful, not just for training purposes but also to increase understanding of the length and complexity of molecular diagnostics. The videos are not a substitution for vigilant laboratory practices and following SOPs, but function as a visual guide of laboratory steps and operations. Though the choice of reagents, equipment and commercial kits remains specific to the goals of the project (or local laboratory practices), the principles remain the same. This is an Iba pa series and has received a rating of 0/10 from 0 viewers.
Kung Saan Mapapanood
ugc-edu.comAma Adepa
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Produksyon
Castle Rock Entertainment
Parangal
21 panalo at 43 nominasyon sa kabuuan
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Review ng User
This video demonstrates a tutorial on running a script written in Python (provided by Panos Bozelos), for compiling the individually exported qPCR file outputs, as ‘.xlsx’ files (demonstrated in CLIP4) into a single master file for downstream analysis in R or equivalent.
This video shows: i) the qPCR software set-up for the detection of soil-transmitted helminths, and ii) the review of previous qPCR runs with some troubleshooting. We have used the StepOne Plus instrument from Thermofisher for the demonstration of “Standard curve/Absolute quantitation”, advanced set up. Visit your instrument’s manual for more details and guidance with the set-up for your work.
This video demonstrates: i) the serial dilutions of DNA standards (or positive controls), ii) the loading of DNA samples in the qPCR, 96-well plate, and iii) the sealing and spinning of the qPCR plate. The 96-well template introduced in this video covers the screening of 39 DNA samples in duplicate, DNA standards in triplicate and non-template controls, also in triplicate. Each plate contains the screening of a single target (e.g., single STH species or extraction control). The video contains tips and explanations at each step of the process (Note: instructions are not a substitute for following standard operating procedures). Total duration 14 minutes
This video demonstrates: i) the resuspension of primers/probes used in qPCR testing, ii) the preparation of the master mix for qPCR reactions and the 96-well plate loading, and, iii) the aliquoting of the reagent mix (a mix containing enzyme, salts etc). The video contains tips and explanations at each step of the process (Note: instructions are not a substitute for following standard operating procedures). Total duration 9 minutes
This video demonstrates the extraction of DNA from stool samples, using the FAST SPIN kit for soil from MP Biomedicals. The video contains tips and explanations at each step of the process (Note: instructions are not a substitute for following standard operating procedures). Total duration 42 minutes
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